Journal: bioRxiv

Article Title: mTORC1 signaling is not essential for the maintenance of muscle mass and function in adult sedentary mice

doi: 10.1101/738294

Figure Lengend Snippet: Removal of mTORC1 leads to a reduction in translational components. (A) Western blot of an m7GTP pulldown on gastrocnemius lysates of 3-month iRAmKOs with the primary antibodies against eIF4E and 4E-BP1. (B) Quantification of the m7GTP pulldown. The levels of 4E-BP1 were normalised to the amount of eIF4E. (C) Average relative expression levels of the ribosomal proteins obtained from the MS data, separated into 40S and 60S subunits. (D) Significantly decreased proteins involved in translation from the MS data (excluding false discovery rate). (E) Average polysome profiles of gastrocnemius from 3-month iRAmKOs and controls. The monosomes are labelled as 80S. 3-month iRAmKOs and controls n = 3. Values represent the mean ± SEM. Significance was assessed using two-tailed unpaired student’s t -test: *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Next, twenty μl of a 50% slurry of m7GTP-Agarose beads (Jena Bioscience) was added to the supernatant overnight at 4°C.

Techniques: Western Blot, Expressing, Two Tailed Test